Journal: Cancer Research Communications

Article Title: 3D “Emboli” Culture Models Epithelial Breast Cancer Cell Oxidative Mitochondrial Metabolism with Relevance for Lung Metastasis

doi: 10.1158/2767-9764.CRC-25-0587

Figure Lengend Snippet: EmC leads to induction of genes related to lung metastasis. A, qRT-PCR of mRNA levels for indicated genes in SUM149 and MDA-MB-468 cells under the indicated culture conditions ( n = 3). B, Differential expression of the indicated genes derived from the scRNA-seq data comparing EmC vs. SphC in SUM149 and IBC-3 cells as indicated. C, qRT-PCR (top, n = 3) of ID1 expression in SUM149 and MDA-MB-468 cultures as indicated. D, Western blot analysis of ID1 expression of cells as in C . Actin served as a loading control. E, Western blot analysis of ID1 and ID3 proteins in established SUM149 EmC cells that were treated with AGX51 at the indicated concentrations for 3 days. Actin served as a loading control. F, FC in viable SUM149 and MDA-MB-468 cells in established EmC following AGX51 treatment at the indicated concentrations for 3 days ( n = 3). G, FC in viable SUM149 and MDA-MB-468 cells in established EmC and SphC following AGX51 treatment (100 μmol/L) for 3 days ( n = 3). Quantitative data are shown as mean ± SEM, *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; n.s., not significant.

Article Snippet: Immunohistochemistry was performed according to standard procedures with primary antibodies for ID1 at 1:50 (Cell Signaling Technology #23369), Ki67 at 1:200 (Cell Signaling Technology #9027), or isotype control rabbit monoclonal IgG (Cell Signaling Technology # 3900).

Techniques: Quantitative RT-PCR, Quantitative Proteomics, Derivative Assay, Expressing, Western Blot, Control

Journal: Scientific Reports

Article Title: The role of cellular senescence-related genes in ischemia–reperfusion injury and the identification of their biomarkers

doi: 10.1038/s41598-026-36076-2

Figure Lengend Snippet: Internal validation of combined IRI diagnostic model. ( a ) Nomogram of Model Genes in the diagnostic model of IRI in the combined GEO dataset. ( b ) DCA plot of the Model Genes in the Combined GEO Dataset for the Diagnostic Model of IRI. ( c ) ROC curve of the RiskScore of the IRI diagnostic model from the combined GEO dataset. D-I. ROC curves for CDKN2B ( d ), ID1 ( e ), STAT3 ( f ), TERF2 ( g ), TP53 ( h ), and ZNF277 ( i ) were analyzed between disease control groups using the combined GEO dataset. AUC < 0.9 indicated high accuracy, 0.7 < AUC0.9 indicated substantial accuracy, and 0.5 < AUC < 0.7 indicated lower accuracy.

Article Snippet: The slides were incubated with primary antibodies: anti-CDKN2B (1:50, Biodragon, China), TP53 (1:50, ProteinTech Group, China), TERF2 (1:50, ProteinTech Group, China), STAT3 (1:600, Servicebio, China), and ID1 (1:800, Servicebio, China).

Techniques: Biomarker Discovery, Diagnostic Assay, Control

Journal: Scientific Reports

Article Title: The role of cellular senescence-related genes in ischemia–reperfusion injury and the identification of their biomarkers

doi: 10.1038/s41598-026-36076-2

Figure Lengend Snippet: In vivo verification of targeted genes in IRI mice. ( a ) Workflow of experimental design. ( b - g ) mRNA levels of CDKN2B , ID1 , STAT3 , TP53 , TERF2 , and ZNF277 were assessed at different time points in mice model of IRI. ( h ) IHC of 5 model genes with AUC > 0.9. Bar = 50 μm. ( i - m ) Statistical plot of average optical density values of IHC for model genes ( CDKN2B , TP53 , TERF2 , STAT3 , and ID1 ). * p < 0.05, **p < 0.01, and *** p < 0.001.

Article Snippet: The slides were incubated with primary antibodies: anti-CDKN2B (1:50, Biodragon, China), TP53 (1:50, ProteinTech Group, China), TERF2 (1:50, ProteinTech Group, China), STAT3 (1:600, Servicebio, China), and ID1 (1:800, Servicebio, China).

Techniques: In Vivo